Unlocking Nature's Secrets: The Anti-Cancer Potential of Cantharellus cibarius
Explore the remarkable findings on small RNAs from Cantharellus cibarius that exhibit selective anti-cancer properties, revealing their potential in targeting tumor cells while sparing normal ones.
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Prompt
Edible mushrooms are widely recognized for their nutraceutical properties, including potential anti-cancer activity. Recent studies have highlighted small RNAs (sRNAs) isolated from Cantharellus cibarius as bioactive molecules capable of inhibiting tumor cell proliferation. This study’s aim is to evaluate the anti-cancer potential of sRNA-enriched fractions isolated from Cantharellus cibarius. RNA fractions were obtained through anion exchange chromatography, and their RNA nature was confirmed by RNAse digestion and migration in denaturing gel electrophoresis. The anti-proliferative activity of these fractions was assessed in Caco-2 (tumor) and HDFn (normal) cell lines using MTT assays. Using anion exchange chromatography, it was possible to isolate a fraction enriched in nucleic acids. This fraction showed to be RNA-enriched by the absence of nucleic acid after RNase digestion and a size above 100 base pairs. Results demonstrated that the RNA-enriched fraction significantly reduced the viability of Caco-2 cells while having minimal effects on HDFn cells, suggesting selective anti-cancer potential. After purification with RNA-specific columns, the anti-cancer effect was highly reduced, suggesting that this sRNA-enriched fraction also contains a molecule that facilitates cancer cell penetration. These findings support the potential of C. cibarius as a source of bioactive RNAs with therapeutic relevance. Further studies should focus on identifying the specific RNA sequence responsible for this effect, as well as its target, and exploring their mechanisms of action in cancer therapy.
