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π Biofuel Battle: Cultivate, Compete, Conquer!
Join the 3-Week Challenge to grow Nannochloropsis salina in your own mini-photobioreactor, optimize biodiesel production, and compete for awards while mastering real-world biotechnology!
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Prompt
π±β¨ Grow Your Fuel! Biodiesel From Nannochloropsis salina 3-Week Student Challenge ________________________________________ π¬π§ͺ What You Will Do β’ πΏ Grow N. salina in your own miniβphotobioreactor. β’ π Measure optical density (OD) and track growth. β’ π‘ Adjust light, nutrients, and aeration to boost productivity. β’ π§΄ Perform the SPV lipid assay to measure oil content. β’ π Compete with other teams to create the best biodieselβready algae culture! ________________________________________ βπ Your Mission Maximize biodiesel potential by optimizing: - π Biomass β how much algae you grow - π‘ Lipids β how much oil the algae produce π― Final Score = 0.6 Γ Lipid Score + 0.4 Γ Biomass Score ________________________________________ π ποΈ Weekly Schedule Week 1 β π§« Setup & Early Growth Intro β’ Inoculate cultures β’ Baseline OD & pH β’ Choose strategy Week 2 β π Optimization & Lipid Induction Measure OD & pH β’ Adjust light/nutrients/aeration β’ Apply nitrogen limitation β’ First SPV lipid test Week 3 β π Final Measurements & Competition Final OD β’ Final SPV lipid assay β’ Calculate biodiesel score β’ Present results β’ Awards ________________________________________ π§ π‘ Tips for Success β’ π Record all measurements carefully. β’ π§ Change only one variable at a time. β’ π¨ Nitrogen limitation increases lipids (but donβt starve them!). β’ π Think like a scientist: plan β test β analyze β improve. β’ π Observe color changes β darker green often means healthier biomass. ________________________________________ ππ₯ Awards β’ π Best Biodiesel Potential β’ π± Best Growth β’ π Best Lab Notebook β’ π‘ Most Innovative Strategy β’ π§Ό Cleanest Lab Team ________________________________________ ππ Welcome to the Challenge! Youβre about to do real biotechnology β growing, measuring, optimizing, and competing. Good luck, and may the best microalgae team win!